Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation

The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge...

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Published inPeerJ preprints
Main Authors Odoni, Dorett I, Tamayo-Ramos, Juan A, Sloothaak, Jasper, VanHeck, Ruben G A, Vitor A P Martins dos Santos, de Graaff, Leo H, Suarez-Diez, Maria, Schaap, Peter J
Format Journal Article
LanguageEnglish
Published San Diego PeerJ, Inc 23.01.2017
Subjects
Amino acids
Metabolic pathways
Proteomics
Urea
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Summary:The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
ISSN:2167-9843
DOI:10.7287/peerj.preprints.2742v1