Haploid androgenetic development in bovines reveals imbalanced WNT signaling and impaired cell fate differentiation

Haploid embryos have contributed significantly to our understanding of the role of parental genomes in development and can be applied to important biotechnology for human and animal species. However, development to the blastocyst stage is severely hindered in bovine haploid androgenetic embryos (hAE...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Aguila, Luis, Nociti, Ricardo P, Rafael Vilar Sampaio, Therrien, Jacinthe, Flavio Viera Meirelles, Ricardo Nicolas Felmer, Smith, Lawrence Charles
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 28.01.2023
Subjects
Biotechnology
Blastocysts
CDX2 protein
Cell differentiation
Cell fate
Developmental stages
Embryos
Genomes
KLF4 protein
Oct-4 protein
Pluripotency
Signal transduction
Transcriptomics
Wnt protein
Online AccessGet full text

Cover

More Information
Summary:Haploid embryos have contributed significantly to our understanding of the role of parental genomes in development and can be applied to important biotechnology for human and animal species. However, development to the blastocyst stage is severely hindered in bovine haploid androgenetic embryos (hAE). To further our understanding of such developmental arrest, we performed a comprehensive comparison of the transcriptomic profile of morula-stage embryos, which were validated by qRT-PCR of transcripts associated with differentiation in haploid and biparental embryos. Among numerous disturbances, results showed that pluripotency pathways, especially the wingless-related integration site (WNT) signaling, were particularly unbalanced in hAE. Moreover, transcript levels of KLF4, NANOG, POU5F1, SOX2, CDX2, CTNNBL1, AXIN2, and GSK3B were noticeably altered in hAE, suggesting disturbance of pluripotency and canonical WNT pathway. To evaluate the role of WNT on hAE competence, we exposed early day-5 morula stage embryos to the GSK3B inhibitor CHIR99021. Although no alterations were observed in pluripotency and WNT-related transcripts, exposure to CHIR99021 improved their ability to reach the blastocysts stage, confirming the importance of the WNT pathway in the developmental features of bovine hAE.Competing Interest StatementThe authors have declared no competing interest.
DOI:10.1101/2023.01.27.525928