Translate the untranslated: excising as a novel post-splicing event

Maturing of a messenger RNA (mRNA) is a multi-way process producing mRNA variants through diverse splicing events, alternative polyadenylation, RNA editing, etc. Studying post-transcriptional processing of human cold-inducible RNA binding protein (CIRBP), we discovered yet another mechanism that cou...

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Bibliographic Details
Published inbioRxiv
Main Authors Panteleev, Dmitry Y, Reshetnikov, Roman V, Samoylenkova, Nadezhda S, Pustogarov, Nikolay A, Pavlova, Galina V
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 07.02.2020
Subjects
3' Untranslated regions
Alternative splicing
Clonal deletion
Cloning vectors
Polyadenylation
Post-transcription
RNA editing
RNA processing
RNA-binding protein
Structure-function relationships
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Summary:Maturing of a messenger RNA (mRNA) is a multi-way process producing mRNA variants through diverse splicing events, alternative polyadenylation, RNA editing, etc. Studying post-transcriptional processing of human cold-inducible RNA binding protein (CIRBP), we discovered yet another mechanism that could be added to this list. We named it excising, and it consists of low-accuracy post-splicing deletion of sequence regions of variable length. The main features of the excising process and putative members of corresponding multiprotein machinery were described with a series of cloning vectors and RNA-pulldown assay. Our results highlight a possible role of U-rich stretches and the proteins targeting such motifs in the discovered process. The discovered mechanism suggests the potential translation of 3'-untranslated regions, which may be an adjuvant way of CIRBP activity inhibition or generation of structural and functional diversity.
DOI:10.1101/2020.02.07.938738