Minor pilin genes are involved in motility and natural competence in Synechocystis sp. PCC 6803

• Published in: bioRxiv
• Main Authors: Oeser, Sabrina, Wallner, Thomas, Bučinská, Lenka, Bähre, Heike, Schürgers, Nils, Wilde, Annegret
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 15.12.2020
• Subjects: Cell surface; Conflicts of interest; DNA microarrays; Flocculation; Gene expression; Genetic transformation; Motility; Pilin; Synechocystis; Transcription
• DOI: 10.1101/2020.12.15.422378

Abstract Cyanobacteria synthesize type IV pili, which are known to be essential for motility, adhesion and natural competence. They consist of long flexible fibres that are primarily composed of the major pilin PilA1 in Synechocystis sp. PCC 6803. In addition, Synechocystis encodes less abundant pilin-like proteins, which are known as minor pilins. The transcription of the minor pilin genes pilA5, pilA6 and pilA9-pilA11 is inversely regulated in response to different conditions. In this study, we show that the minor pilin PilA5 is essential for natural transformation but is dispensable for motility and flocculation. In contrast, a set of minor pilins encoded by the pilA9-slr2019 transcriptional unit are necessary for motility but are dispensable for natural transformation. Neither pilA5-pilA6 nor pilA9-slr2019 are essential for pilus assembly as mutant strains showed type IV pili on the cell surface. Microarray analysis demonstrated that the transcription levels of known and newly predicted minor pilin genes change in response to surface contact. A total of 120 genes were determined to have altered transcription between planktonic and surface growth. Among these genes, 13 are located on the pSYSM plasmid. The results of our study indicate that different minor pilins facilitate distinct pilus functions. Competing Interest Statement The authors have declared no competing interest. Footnotes * Funding information: This work was supported by grants to AW of the German Science Foundation (DFG) as part of DFG Priority Programme SPP1879 (Wi 2014/7-1) and the SFB1381 (A2). * Conflict of interest statement: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. * Data availability statement: The data that support the findings of this study are openly available in NCBI’s Gene Expression Omnibus at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE161586, reference number GSE161586.