CULTURE AND MAINTENANCE OF URINE-DERIVED, 3-DIMENSIONAL CANINE TRANSITIONAL CELL CARCINOMA ORGANOIDS

Bladder cancer is the ninth most common malignancy in the world. Transitional cell carcinoma (TCC), also referred to as urothelial carcinoma (UC) is the most common form of bladder cancer, occurring in 90% of cases. In this study, we explore urine-derived, 3-dimensional, canine TCC organoids as a po...

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Published inbioRxiv
Main Authors Thenuwara, Savantha, Schneider, Benjamin, Mosichuk, Allison, Gabriel, Vojtech, Zdyrski, Christopher, Dao, Kimberly, Iennarella-Servantez, Chelsea, Colosimo, Madeline, Sahoo, Dipak, Bourgois-Mochel, Agnes, Musser, Margaret, Johannes, Chad, Jean-Sebastien Palerme, Allenspach, Karin, Mochel, Jonathan P
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 24.08.2021
Subjects
Bladder cancer
Cell culture
Cell viability
Cisplatin
Hybridization
Malignancy
Organoids
Ribonucleic acid
RNA
Transitional cell carcinoma
Tumors
Urine
Urothelial carcinoma
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Summary:Bladder cancer is the ninth most common malignancy in the world. Transitional cell carcinoma (TCC), also referred to as urothelial carcinoma (UC) is the most common form of bladder cancer, occurring in 90% of cases. In this study, we explore urine-derived, 3-dimensional, canine TCC organoids as a possible model to study bladder TCC ex vivo. After establishing the cell lines, we subjected the 3D cells to RNA in situ hybridization (RNA-ISH) and cell viability assays. Overall, 3D cell culture from urine samples of TCC diagnosed canines expressed RNA biomarkers in a similar manner to parent tumors via RNA-ISH and showed more sensitivity to cisplatin treatment when compared to 2D human TCC cells. With further experimentation, canine TCC organoids could become an ideal model to study TCC ex vivo. Competing Interest Statement Mochel and Allenspach are co-founders of 3D Health Solutions, a spin-off from Iowa State University focusing on drug testing with 3D organoids. Dao is an employee of 3D Health Solutions.
DOI:10.1101/2021.08.24.457548