Single-nucleus RNA-seq2 reveals a functional crosstalk between liver zonation and ploidy

Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity we have developed a single-nucleus RNA-seq-2 method that allows deep characterization of nuclei isolated from frozen arch...

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Bibliographic Details
Published inbioRxiv
Main Authors Richter, Maria L, Deligiannis, Ioannis K, Danese, Anna, Lleshi, Ermira, Vallejos, Catalina A, Coupland, Paul, Colome-Tatche, Maria, Martinez-Jimenez, Celia P
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 12.07.2020
Subjects
Gene dosage
Gene expression
Hepatocytes
Nuclei
Ploidy
Ribonucleic acid
RNA
Spatial distribution
Transcription
Zonation
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Summary:Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity we have developed a single-nucleus RNA-seq-2 method that allows deep characterization of nuclei isolated from frozen archived tissues. We have used this approach to characterize the transcriptional profile of individual hepatocytes with different levels of ploidy, and have discovered that gene expression in tetraploid mononucleated hepatocytes is conditioned by their position within the hepatic lobe. Our work has revealed a remarkable crosstalk between gene dosage and spatial distribution of hepatocytes. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2020.07.11.193458