A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types

Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. To facilitate further cellular investigations, no...

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Published inbioRxiv
Main Authors Miserey-Lenkei, Stéphanie, Trajkovic, Katarina, D'ambrosio, Juan Martìn, Patel, Amanda J, Čopič, Alenka, Mathur, Pallavi, Schauer, Kristine, Goud, Bruno, Albanèse, Véronique, Gautier, Romain, Subra, Melody, Kovacs, David, Barelli, Hélène, Antonny, Bruno
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 19.12.2020
Subjects
Cell culture
Cell lines
Endosomes
Epithelial cells
Localization
Mitochondria
Plasmids
Protein interaction
Proteins
Severe acute respiratory syndrome coronavirus 2
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Summary:Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. To facilitate further cellular investigations, notably by imaging techniques, we present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterization in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localization of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localization of Nsp13 to the centrosome, Orf3a to late endosomes, and Orf9b to mitochondria. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2020.12.19.423586