Dysregulated gene expression of imprinted and X-linked genes: a link to poor development of bovine haploid androgenetic embryos

Abstract Mammalian uniparental embryos are efficient models for genome imprinting research and allow studies on the contribution of the paternal and maternal genome to early embryonic development. In this study, we analyzed different methodologies for production of bovine haploid androgenetic embryo...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Aguila, Luis, Therrien, Jacinthe, Suzuki, Joao, García, Mónica, Trindade, Amanda, Smith, Lawrence C
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 24.10.2020
Subjects
Developmental stages
Embryogenesis
Enucleation
Epigenetics
Gene expression
Genomes
Genomic imprinting
Haploidy
KCNQ1 protein
KCNQ1OT1 protein
Methyltransferase
Non-coding RNA
Potassium channels (voltage-gated)
X-chromosome inactivation
Online AccessGet full text

Cover

More Information
Summary:Abstract Mammalian uniparental embryos are efficient models for genome imprinting research and allow studies on the contribution of the paternal and maternal genome to early embryonic development. In this study, we analyzed different methodologies for production of bovine haploid androgenetic embryos (hAE) to elucidate the causes behind their poor developmental potential. The results showed that hAE can be efficiently generated by using intracytoplasmic sperm injection and oocyte enucleation at telophase II. Although haploidy does not disturb early development up to around the 3rd mitotic division, androgenetic development is disturbed after the time of zygote genome activation those that reach the morula stage are less capable to become a blastocyst. Analysis of gene expression indicated abnormal levels of methyltransferase 3B and key long non-coding RNAs involved in X-chromosome inactivation and genomic imprinting of the KCNQ1 locus, which is associated to the methylation status of imprinted control regions of XIST and KCNQ1OT1. Thus, our results seem to exclude micromanipulation consequences and chromosomal abnormalities as major factors in developmental restriction, suggesting that their early developmental constraint is regulated at an epigenetic level. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2020.10.23.350405