Human neonatal B cell immunity differs from the adult version by conserved Ig repertoires and rapid, but transient response dynamics

• Published in: bioRxiv
• Main Authors: Budeus, Bettina, Kibler, Artur, Brauser, Martina, Homp, Ekaterina, Bronischewski, Kevin, Ross, J Alexander, Görgens, Andre, Weniger, Marc A, Dunst, Josefine, Kreslavsky, Taras, Symone Vitoriano Da Conceição Castro, Murke, Florian, Oakes, Christopher C, Rusch, Peter, Andrikos, Dimitrios, Kern, Peter, Köninger, Angela, Lindemann, Monika, Johansson, Patricia, Hansen, Wiebke, Lundell, Anna-Carin, Rudin, Anna, Dürig, Jan, Giebel, Bernd, Hoffmann, Daniel, Küppers, Ralf, Seifert, Marc
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 10.09.2020
• Subjects: Animal models; B-cell receptor; Cell differentiation; Class switching; Immunoglobulin A; Immunological memory; Lymphocytes B; Lymphocytes T; Neonates; Transcription; Vaccination
• DOI: 10.1101/2020.08.11.245985

Abstract The human infant B cell system is considered premature or impaired. Here we show that most cord blood B cells are mature and functional as seen in adults, albeit with distinct transcriptional programs providing accelerated responsiveness to T cell-independent and T cell-dependent stimulation and facilitated IgA class switching. Stimulation drives extensive differentiation into antibody-secreting cells, thereby presumably limiting memory B cell formation. The neonatal Ig-repertoire is highly variable, but conserved, showing recurrent B cell receptor (BCR) clonotypes frequently shared between neonates. Our study demonstrates that cord blood B cells are not impaired but differ from their adult counterpart in a conserved BCR repertoire and rapid but transient response dynamics. These properties may account for the sensitivity of neonates to infections and limited effectivity of vaccination strategies. Humanized mice suggest that the distinctness of cord blood versus adult B cells is already reflected by the developmental program of hematopoietic precursors, arguing for a layered B-1/B-2 lineage system as in mice. Still, our findings reveal overall limited comparability of human cord blood B cells and murine B-1 cells. Significance Statement Neonates and infants suffer from enhanced susceptibility to infections. Our study contrasts with the current concept of a premature or impaired B cell system in neonates, by showing that most cord blood B cells are mature and functional. However, their responses are rapid but provide only short-term protection, which may help to improve infant vaccination strategies. We propose an altered perspective on the early human B cell system, which looks similar to but functions differently from the adult counterpart. Finally, our analysis indicates that cord blood- and adult B cell development occur layered as in mice, but certain mouse models still may offer a limited view on human neonatal B cell immunity. Competing Interest Statement The authors have declared no competing interest. Footnotes * We added a chapter on humanized mice, an additional figure and edited our manuscript file according to peer reviewers suggestions.