Interfacial tension driven open droplet microfluidics

• Published in: bioRxiv
• Main Authors: Jian Wei Khor, Lee, Ulri N, Berthier, Jean, Berthier, Erwin, Theberge, Ashleigh B
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 14.04.2022
• Subjects: Microfluidics
• DOI: 10.1101/2021.07.29.454194

We developed an open channel droplet microfluidic system that autonomously generates droplets at low Ca (~10-4-10-3) by leveraging competing hydrostatic and capillary pressure. With only our open channel polytetrafluoroethylene (PTFE) device, pipettes, and commercially available carrier fluid, we produce hundreds of microliter droplets; tubing, electronics, or pumps are not required, making droplet technology feasible for research labs without external flow generators. Furthermore, we demonstrated conceptual applications that showcase the process of droplet generation, splitting, transport, incubation, mixing, and sorting in our system. Unlike conventional droplet microfluidics, the open nature of the device enables the use of physical tools such as tweezers and styli to directly access the system; with this, we developed a new method of droplet sorting and transfer that capitalizes on the Cheerios effect, the aggregation of buoyant objects along a liquid interface. Our platform offers enhanced usability, direct access to the droplet contents, easy manufacturability, compact footprint, and high customizability. This design is a first step in exploring the space of power-free open droplet microfluidic systems and provide design rules for similar channel designs. Competing Interest Statement ABT: ownership in Stacks to the Future, LLC and Seabright, LLC. EB: ownership in Stacks to the Future, LLC, Salus Discovery, LLC, Seabright, LLC, and Tasso, Inc., and employment by Tasso, Inc. However, this research is not related to these companies.