Residual DNA in enzymes may pose a challenge for DIY RNA sequencing protocols: how to deal

High-throughput RNA sequencing (RNA-seq) is widely employed for gene expression profiling. This report on do-it-yourself RNA-Seq library preparation pitfalls, highlighting their correct identification and successful overcoming, may be helpful to research groups commencing a DIY RNA-Seq workflow and...

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Bibliographic Details
Published inbioRxiv
Main Authors Krivoy, Andrey, Botsmanov, Yegor, Cheranev, Valery, Shmitko, Anna, Belova, Vera, Moskalenko, Viktoriya, Afasizhev, Robert, Shut, Anastasia, Korostin, Dmitriy, Rebrikov, Denis
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 04.02.2022
Subjects
DNA sequencing
Gene expression
Ribonucleic acid
RNA
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Summary:High-throughput RNA sequencing (RNA-seq) is widely employed for gene expression profiling. This report on do-it-yourself RNA-Seq library preparation pitfalls, highlighting their correct identification and successful overcoming, may be helpful to research groups commencing a DIY RNA-Seq workflow and facing similar problems. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2022.02.02.478843