Neural progenitor cell-derived nanovesicles promote hair follicle growth via miR-100

• Published in: Journal of Nanobiotechnology
• Main Authors: Cao, Lei, Tian, Tian, Huang, Yuanbo, Tao, Shiqin, Zhu, Xiaohong, Yang, Mifang, Gu, Jing, Feng, Guangdong, Ma, Yinni, Xia, Rushan, Xu, Wenrong, Wang, Lei
• Format: Web Resource
• Language: English
• Published: Durham Research Square 08.12.2020
• DOI: 10.21203/rs.3.rs-50127/v2

Background: Accumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth. However, large-scale production of EVs is still a challenge. Recently, exosome-mimetic nanovesicles (NV) prepared by extruding cells have emerged as an alternative strategy for clinical-scale production. Here, ReNcell VM (ReN) cells, a neural progenitor cell line was serially extruded to produce NV. Results: The ReN-NV were found to promote dermal papilla cell (DPC) proliferation. In addition, in a mouse model of depilation-induced hair regeneration, ReN-NV were injected subcutaneously, resulting in an acceleration of hair follicle (HF) cycling transition at the site. The underlying mechanism was indicated to be the activation of Wnt/β-catenin signaling pathway. Furthermore, miR-100 was revealed to be abundant in ReN-NV and significantly up-regulated in DPCs receiving ReN-NV treatment. miR-100 inhibition verified its important role in ReN-NV-induced β-catenin signaling activation. Conclusion: These results provide an alternative agent to EVs and suggest a strategy for hair growth therapy.