Multi-virulence and phenotypic spread of Campylobacter jejuni carried by chicken meat in Brazil

• Published in: bioRxiv
• Main Authors: Phelipe Augusto Borba Martins Peres, Torres De Melo, Roberta, Armendaris, Paulo Marcel, Barreto, Fabiano, Tiago Follmann Perin, Grazziotin, Ana Laura, Guilherme Paz Monteiro, Eliane Pereira Mendonça, Eduarda Cristina Alves Lourenzatto, Arthur Slompo Muniz Bicalho, Marcelo De Vito Filho, Ana Beatriz Garcez Buiatte, Daise Aparecida Rossi
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 14.12.2021
• Subjects: Adaptation; Biofilms; Campylobacter; Campylobacter jejuni; Carcasses; Cytotoxicity; Disease hot spots; Environmental conditions; Epidemiology; Gastroenteritis; Genes; Meat; Proteomics; Public health; Strains (organisms); Virulence; Brazil
• DOI: 10.1101/2021.12.14.472542

Campylobacter jejuni is the most incriminated pathogen in bacterial gastroenteritis, and therefore, characteristics of its epidemiology must be continuously investigated to support possible mitigating measures. This is particularly important when evaluating representative strains of the world's leading chicken meat exporter, Brazil. We evaluated a panel of 14 virulence genes in 359 strains of C. jejuni isolated from chilled broiler carcasses of Brazil. The genes were classified into five virulence categories (B: biofilm/motility; SS: secretion/cytotoxicity system; CI: invasion/colonization; GB: Guillain-Barré and AE: adaptation to stress). The percentage of strains with stress adaptation genes (86.07%) indicates the potential to adapt to unfavorable environmental conditions and hcp gene in 97.77%, indicates the ability to cause serious infections in humans. Genes related to GBS in 77.44% of strains are an additional concern, which must be monitored. The gene panel showed the presence of 124 virulence profiles. Individual analyzes by carcass, slaughter establishment, and municipalities where they were located showed high I.Var., of 0.82, 0.87 and 0.78, respectively. Georeferencing indicated state A as a hotspot for virulent strains. Higher levels of isolation and multi-virulence were identified in the summer, which in Brazil is hot and humid. Proteomics was able to discriminate the strains, but due to the high heterogeneity between them, it did not allow to explain their dissemination. Together, our results showed that the studied strains are a potential danger to public health and that there is an urgent need for their surveillance and the adoption of control measures, especially in state A. Competing Interest Statement The authors have declared no competing interest.