Combined analysis of the time-resolved transcriptome and proteome of plant pathogen Xanthomonas oryzae pv. oryzae

Abstract Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen responsible for causing bacterial blight in rice. The immediate alterations in Xoo upon initial contact with rice are essential for pathogenesis. We studied time-resolved genome-wide gene expression in pathogenicity-activated Xoo cells...

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Bibliographic Details
Published inbioRxiv
Main Authors Kim, Seunghwan, Jang, Wooyoung Eric, Min-Sik, Kim, Jeong-Gu, Kim, Lin-Woo, Kang
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 30.01.2021
Subjects
Blight
Gene expression
Genomes
Liquid chromatography
Mass spectroscopy
Pathogenesis
Pathogenicity
Pathogens
Proteomes
Rice
Transcription
Transcriptomes
Translation
Xanthomonas oryzae
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Summary:Abstract Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen responsible for causing bacterial blight in rice. The immediate alterations in Xoo upon initial contact with rice are essential for pathogenesis. We studied time-resolved genome-wide gene expression in pathogenicity-activated Xoo cells at the transcriptome and proteome levels. The early response genes of Xoo include genes related to cell motility, inorganic ion transport, and effectors. The alteration of gene expression is initiated as early as few minutes after the initial interaction and changes with time. The time-resolved comparison of the transcriptome and proteome shows the differences between transcriptional and translational expression peaks in many genes, although the overall expression pattern of mRNAs and proteins is conserved. The discrepancy suggests an important role of translational regulation in Xoo at the early stages of pathogenesis. The gene expression analysis using time-resolved transcriptome and proteome provides unprecedented valuable information regarding Xoo pathogenesis. Competing Interest Statement The authors have declared no competing interest. Footnotes * The manuscript is revised for clarity and English was corrected. Fig 7 is added. * Abbreviations Xoo Xanthomonas oryzae pv. oryzae RLX rice leaf homogenate KACC Korean Agricultural Collection Center P-activated Pathogenicity-activated LC-MS/MS liquid chromatography-tandem mass spectrometry COG Clusters of Orthologous Groups RPKM Reads per kilobase per million mapped reads
DOI:10.1101/2020.10.02.324541