Lipid fingerprints are similar between SLC6 transporters in the neuronal membrane

ABSTRACT We use molecular dynamics simulations to characterise the local lipid annulus, or “fingerprint”, of three SLC6 transporters (dDAT, hSERT, and GlyT2) embedded into a complex neuronal membrane. New membrane analysis tools were created to improve leaflet detection and leaflet-dependent propert...

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Bibliographic Details
Published inbioRxiv
Main Authors Wilson, Katie A, Wang, Lily, Yie Chang Lin, Megan L O’mara
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 21.01.2021
Subjects
Cholesterol
Embedding
Glycine transporter
Lipids
Membrane composition
Membrane proteins
Protein interaction
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Summary:ABSTRACT We use molecular dynamics simulations to characterise the local lipid annulus, or “fingerprint”, of three SLC6 transporters (dDAT, hSERT, and GlyT2) embedded into a complex neuronal membrane. New membrane analysis tools were created to improve leaflet detection and leaflet-dependent properties. Overall, lipid fingerprints are comprised of similar lipids when grouped by headgroup or tail saturation. The enrichment and depletion of specific lipids, including sites of cholesterol contacts, varies between transporters. The subtle differences in lipid fingerprints results in varying membrane biophysical properties near the transporter. Through comparisons to previous literature, we highlight that the lipid-fingerprint in complex membranes is highly dependent on membrane composition. Furthermore, through embedding these transporters in a simplified model membrane, we show that the simplified membrane is not able to capture the biophysical properties of the complex membrane. Our results further characterise how the presence and identity of membrane proteins affects the complex interplay of lipid-protein interactions, including the local lipid environment and membrane biophysical properties. HIGHLIGHTS * Lipid fingerprints are comprised of similar lipid classes * Sites of specific lipid contacts, including CHOL, varies between transporters * Changes in lipid annulus result in variable local membrane biophysical properties * Membrane composition, including that of complex membranes, affects lipid annulus Figure1 Figure1 * Download figure * Open in new tab Competing Interest Statement The authors have declared no competing interest. Footnotes * ↵# Co-first author * https://github.com/OMaraLab/SLC6_lipid_fingerprints
DOI:10.1101/2021.01.20.427530