Diabetes induced renal urea transport alterations assessed with 3D hyperpolarized 13C,15N‐Urea

Purpose In the current study, we investigated hyperpolarized urea as a possible imaging biomarker of the renal function by means of the intrarenal osmolality gradient. Methods Hyperpolarized three‐dimensional balanced steady state 13C MRI experiments alongside kidney function parameters and quantita...

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Published inMagnetic resonance in medicine Vol. 77; no. 4; pp. 1650 - 1655
Main Authors Bertelsen, Lotte B., Nielsen, Per M., Qi, Haiyun, Nørlinger, Thomas S., Zhang, Xiaolu, Stødkilde‐Jørgensen, Hans, Laustsen, Christoffer
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc 01.04.2017
Subjects
hyperpolarization
kidney
MRI
renal function
Type 1 diabetes
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Summary:Purpose In the current study, we investigated hyperpolarized urea as a possible imaging biomarker of the renal function by means of the intrarenal osmolality gradient. Methods Hyperpolarized three‐dimensional balanced steady state 13C MRI experiments alongside kidney function parameters and quantitative polymerase chain reaction measurements was performed on two groups of rats, a streptozotocin type 1 diabetic group and a healthy control group. Results A significant decline in intrarenal steepness of the urea gradient was found after 4 weeks of untreated insulinopenic diabetes in agreement with an increased urea transport transcription. Conclusion MRI and hyperpolarized [13C,15N]urea can monitor the changes in the corticomedullary urea concentration gradients in diabetic and healthy control rats. Magn Reson Med 77:1650–1655, 2017. © 2016 International Society for Magnetic Resonance in Medicine
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.26256