POLYPHENOLS DISTRIBUTION AND RESERVE SUBSTANCES ANALYSIS IN CACAO SOMATIC EMBRYOGENESIS

In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative...

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Published inActa biológica colombiana Vol. 21; no. 2; pp. 335 - 345
Main Authors Adriana María Gallego Rúa, Henao Ramírez, Ana María, Aura Inés Urrea Trujillo, Garcés, Lucía Atehortúa
Format Journal Article
LanguagePortuguese
Spanish
Published Bogota Universidad Nacional de Colombia 01.05.2016
Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
Subjects
BIOLOGY
histology
antioxidants
recalcitrance
somatic embryogenesis
reserve accumulation
histología
recalcitrancia
embriogénesis somática
antioxidantes
acumulación de reservas
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Summary:In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step. Análisis de distribución de polifenoles y sustancias de reserva en embriogénesis somática de cacao
ISSN:0120-548X
1900-1649
DOI:10.15446/abc.v21n2.50196