Chlorantraniliprole induces up-regulated expression of cytochrome P450 genes in Spodoptera exigua (Lepidoptera: Noctuidae)
[Aim] This study aims to understand the induction of chlorantranilprole, a novel diamide insecticide, on the expression of cytochrome P450 genes in the midgut of Spodoptera exigua (Hubner) larvae. [Methods] The 4th instar larvae of S. exigua were fed on artificial diets containing 0.01 and 0.02 mg/k...
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Published in | K'un ch'ung hsueh pao Vol. 58; no. 3; pp. 281 - 287 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
01.01.2015
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Subjects | |
Online Access | Get full text |
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Summary: | [Aim] This study aims to understand the induction of chlorantranilprole, a novel diamide insecticide, on the expression of cytochrome P450 genes in the midgut of Spodoptera exigua (Hubner) larvae. [Methods] The 4th instar larvae of S. exigua were fed on artificial diets containing 0.01 and 0.02 mg/kg of chlorantraniliprole, respectively, until their molting, and the activities of O-deethylase of P450 and the mRNA expression levels of four P450 genes (CYP9A9, CYP4S11, CYP4G37 and CYP6B) and one NADPH cytochrome P450 reductase gene (HQ852049) in the midgut of the 5th instar larvae in 6 to 36 h post treatment were detected by assaying O-deethylation of ethoxyresorufin and quantitative real-time PCR, respectively. [Results] The cytochrome P450 ethoxycoumarin-O-deethylase activity in the 0.02 mg/kg treatment group increased to 1.90 - 2.92-fold as high as the untreated control, while that in 0.01 mg/kg treatment group was only 1.11 - 1.62-fold as high as that of the control. The relative expression levels of CYP9A9 |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0454-6296 |
DOI: | 10.16380/j.kcxb.2015.03.007 |