Sensitive quantification and visual detection of bacteria using CdSe/ZnS[at]SiO sub(2) nanoparticles as fluorescent probes

• Published in: Analytical methods Vol. 6; no. 17; pp. 6802 - 6808
• Main Authors: Wang, Renjie, Xu, Yi, Jiang, Yan, Chuan, Na, Su, Xi, Ji, Jingou
• Format: Journal Article
• Language: English
• Published: 01.08.2014
• Subjects: Bacteria; Cadmium selenides; Fluorescence; Intermetallics; Mathematical analysis; Nanoparticles; Salmonella; Visual
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c4ay01257g

CdSe/ZnS[at]SiO sub(2)-NH sub(2) composite nanoparticles (FNPs) were proposed and used as fluorescent markers for bacteria detection. Salmonella typhimuriumacted as representative sample in this paper. It was shown that hydrophobic CdSe/ZnS quantum dots (QDs) were incorporated into SiO sub(2) spheres by using a self-modified reverse-microemulsion technique. FNPs could be successfully covalently conjugated with the bacteria by glutaraldehyde in a two-step strategy. A good linear relationship between the concentration of Salmonella typhimuriumand fluorescence intensity was obtained in the range of 6.6 10 super(2) to 6.6 10 super(4) cfu mL super(-1), and the equation was I= 0.1331 log C- 0.2017 with R super(2) = 0.9974. The detection limit was 3.3 10 super(2) cfu mL super(-1), and this method could be applied to other bacteria detection as well. In order to further reduce the detection limit and achieve better visual determination performance, an integrated dielectrophoresis (DEP) microfluidic chip and relative microsystem was established. The FNP-labeled bacteria could be enriched along the edges of interdigitated microelectrodes in the micro-channel by positive DEP and could be counted under the fluorescence microscope.