Chapter 4 - Electron Microscopy of Bone

Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young an...

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Bibliographic Details
Published inMethods in cell biology Vol. 113; pp. 59 - 79
Main Authors Everts, V, Hoeben, KA
Format Journal Article
LanguageEnglish
Published 01.01.2013
Subjects
Bone
Cartilage
Dentin
Electron microscopy
Mineralization
Sectioning
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Summary:Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young animals, are rather simple to prepare and sectioning is not that much of a problem. In the present chapter we describe step-by-step how to prepare mineralized tissues for ultrastructural examination.
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ISSN:0091-679X
DOI:10.1016/B978-0-12-407239-8.00004-5