Protective effects of a wheat germ peptide (RVF) against H sub(2)O sub(2)-induced oxidative stress in human neuroblastoma cells

RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150-250 mu M, 4 h) and exposed to H sub(2)O sub(2) (200 mu M). RVF increased viable...

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Published inBiotechnology letters Vol. 36; no. 8; pp. 1615 - 1622
Main Authors Cheng, Yunhui, Zhang, Li, Sun, Wei, Tang, Jiqing, Lv, Zhaoliang, Xu, Zhou, Yu, Huixin
Format Journal Article
LanguageEnglish
Published 01.08.2014
Subjects
Biotechnology
Cleavage
Effectiveness
Exposure
Human
Peptides
Pretreatment
Triticum aestivum
Wheat germ
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Summary:RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150-250 mu M, 4 h) and exposed to H sub(2)O sub(2) (200 mu M). RVF increased viable cell numbers by 37 % and reduced the release of lactate dehydrogenase. Pretreatment with RVF also inhibited H sub(2)O sub(2)-induced accumulation of reactive oxygen species and maintained the mitochondrial transmembrane potential as well as preventing intracellular Ca super(2+) dysregulation during H sub(2)O sub(2) exposure. Furthermore, pretreatment with RVF increased the Bcl-2/Bax ratio and blocked cleavage poly(ADP-ribose) polymerase by inhibiting caspase-3 activation, thus decreasing apoptosis.
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ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-014-1521-6