Comparison and Recovery of Escherichia coli and Thermotolerant Coliforms in Water with a Chromogenic Medium Incubated at 41 and 44.5 degree C

The culture medium, CHROMagarECC, which contains chromogenic and fluorogenic substrates for the enzymes, beta -galactosidase and beta -glucuronidase, for the simultaneous detection of coliforms and Escherichia coli in water samples, was modified by the addition of sodium pyruvate, and its efficacy w...

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Bibliographic Details
Published inApplied and environmental microbiology Vol. 65; no. 8; p. 3746
Main Authors Alonso, Jose L, Soriano, Adela, Carabjo, Oscar, Amoros, Inmaculada, Garelick, Hemda
Format Journal Article
LanguageEnglish
Published 01.08.1999
Subjects
Escherichia coli
Sodium pyruvate
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Summary:The culture medium, CHROMagarECC, which contains chromogenic and fluorogenic substrates for the enzymes, beta -galactosidase and beta -glucuronidase, for the simultaneous detection of coliforms and Escherichia coli in water samples, was modified by the addition of sodium pyruvate, and its efficacy was evaluated. River and marine water samples were collected and incubated at 41 and 44.5 degree C, and bacteria were enumerated by the membrane filtration technique. Results showed that incubation at 41 degree C gave the best overall recovery of E. coli, thermotolerant coliforms, and beta -galactosidase-positive thermotolerant coliforms other than E. coli. When the sodium pyruvate-modified CHROMagarECC medium was used, target colonies were confirmed to be E. coli at a rate of 91.5% and beta -galactosidase-positive thermotolerant coliforms other than E. coli of likely fecal origin at a rate of 77.4%.
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ISSN:0099-2240