Calmodulin interacts with the platelet ADP receptor P2Y sub(1)

P2Y sub(1) [P2 (purinergic type-2)-receptor 1] is a G-protein-coupled ADP receptor that regulates platelet activation and ADP-induced Ca super(2+) signalling. Studies using P2Y sub(1)-knockout mice, G sub(q)-deficient mice or P2Y sub(1)- selective inhibitors have previously identified a key role for...

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Bibliographic Details
Published inBiochemical journal Vol. 398; no. 3; pp. 339 - 343
Main Authors Arthur, Jane F, Shen, Yang, Mu, Fi-Tjen, Leon, Catherine, Gachet, Christian, Berndt, Michael C, Andrews, Robert K
Format Journal Article
LanguageEnglish
Published 15.09.2006
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Summary:P2Y sub(1) [P2 (purinergic type-2)-receptor 1] is a G-protein-coupled ADP receptor that regulates platelet activation and ADP-induced Ca super(2+) signalling. Studies using P2Y sub(1)-knockout mice, G sub(q)-deficient mice or P2Y sub(1)- selective inhibitors have previously identified a key role for P2Y sub(1) in pathophysiological thrombus formation at high shear stress. We provide evidence that a positively charged juxtamembrane sequence within the cytoplasmic C- terminal tail of P2Y sub(1) can bind directly to the cytosolic regulatory protein calmodulin. Deletion by mutagenesis of the calmodulin-binding domain of P2Y sub(1) inhibits intracellular Ca super(2+) flux in transfected cells. These results suggest that the interaction of calmodulin with the P2Y sub(1) C-terminal tail may regulate P2Y sub(1)-dependent platelet aggregation.
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ISSN:0264-6021
DOI:10.1042/BJ20060822