AMPEROMETRIC BIOSENSOR MODIFIED WITH MULTIWALLED CARBON NANOTUBES FOR GLUCOSE DETERMINATION

• Published in: Biotechnologia acta Vol. 5; no. 1; p. 53
• Main Authors: Rogaleva, N S, Shkotova, L V, L'vova, O V, Garbuz, V V, Muratov, V B, Duda, T I, Vasil'ev, O O, Korpan, Ya I, Biloivan, O A
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01.01.2012
• ISSN: 1995-5537

To improve the analytical characteristics of amperometric biosensors for glucose determination, aminated and carboxylated multi-walled carbon nanotubes were applied. Attested samples of nanotubes were suspended and used for the modification of amperometric transducers C220AT of DropSens and bio-selective membranes. The glucose oxidase membranes were formed on the surface of working electrode by several methods: covalent cross-linking and co-immobilization with horseradish peroxidase in bovine serum albumin gel in glutaraldehyde vapors; and electropolymerization of poly(3,4-ethylenedioxythiophene) in the conductive polymer film. Electrochemical measurements were performed using the device μStat 200 of DropSens. The developed amperometric biosensors with a membrane based on carbon nanotubes have been shown to be advantageous as compared to those without carbon nanotubes in terms of higher sensitivity, lower working potential, and wider dynamic range of the target analyte detection. A reliable correlation was observed between the results of the proposed biosensor method and those obtained by the high performance liquid chromatography.