Anti IL-17A therapy inhibits bone loss in TNF-[alpha]-mediated murine arthritis by modulation of the T-cell balance
Tumour necrosis factor alpha (TNF-[alpha]) is a major inducer for inflammation and bone loss. Here, we investigated whether interleukin (IL)-17 plays a role in TNF-[alpha]-mediated inflammation and bone resorption. Human TNF-[alpha] transgenic (hTNFtg) mice were treated with a neutralizing anti-IL-1...
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Published in | European journal of immunology Vol. 42; no. 2; pp. 413 - 423 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley Subscription Services, Inc
01.02.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Tumour necrosis factor alpha (TNF-[alpha]) is a major inducer for inflammation and bone loss. Here, we investigated whether interleukin (IL)-17 plays a role in TNF-[alpha]-mediated inflammation and bone resorption. Human TNF-[alpha] transgenic (hTNFtg) mice were treated with a neutralizing anti-IL-17A antibody and assessed for inflammation, cartilage and bone damage. T-cell transcription factors and lymphokine patterns were measured in the LNs. IL-17A inhibition in the absence of IL-1 was also evaluated by treating hTNFtg/IL-1-/- mice with an IL-17A neutralizing antibody. IL-17A neutralization had only minor effects on TNF-[alpha]-induced inflammation but effectively reduced local and systemic bone loss by blocking osteoclast differentiation in vivo. Effects were based on a shift to bone-protective T-cell responses such as enhanced Th2 differentiation, IL-4 and IL-12 expression and Treg cell numbers. Whereas inflammation in hTNFtg/IL-1-/- mice was highly sensitive to IL-17A blockade, no shift in the T-cell lineages and no additional benefit on bone mass were observed in response to IL-17A neutralization. We thus conclude that IL-17A is a key mediator of TNF-[alpha]-induced bone loss by closely interacting with IL-1 in blocking bone protective T-cell responses. [PUBLICATION ABSTRACT] |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-2980 1521-4141 |
DOI: | 10.1002/eji.201141871 |