脂滴包被蛋白5对高糖高脂诱导的小鼠心脏微血管内皮细胞凋亡的影响及机制

• Published in: Jie fang jun yi xue za zhi Vol. 42; no. 12; pp. 1045 - 1050
• Main Authors: DU, Jin, Juan-ni, HOU, LI, Xiu-chuan, YANG, Yi, FENG, Jian, CHEN, Sha, Yong-jian, YANG, Hai-feng, PEI
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2017; 610083成都 成都军区总医院心血管内科%610083成都,成都军区总医院心血管内科%610083成都 成都军区总医院心血管内科; 646000四川泸州 西南医科大学临床医学院; 400038重庆 陆军军医大学(第三军医大学)研究生管理大队
• Subjects: Acids; Apoptosis; Glucose; Nitric oxide; Rodents; perilipin-5; 脂滴包被蛋白5; 氧化应激; 高脂; diabetes mellitus; 糖尿病; high fat; oxidative stress
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2017.12.04

Objective To investigate the effects and mechanisms of perilipin-5 (Plin5) on the apoptosis of mouse cardiac microvascular endothelial cells induced by high fat and high glucose. Methods The mouse cardiac microvascular endothelial cells (MCMECs) cultured with high glucose medium were respectively given 0, 100, 300 and 500μmol/L palmitic acid for 24 hours. In order to explore the effects and mechanisms of Plin5 on MCMECs injuries induced by high fat and high glucose, MCMECs exposed to 300μmol/L palmitic acid for 24 hours were divided into control group, Scra siRNA group and Plin5 siRNA group. The control group was only treated with transfection reagent, the Scra siRNA group was given treatment of transfection reagent and garbled RNA, the Plin5 siRNA group was given treatment of transfection reagent and Plin5 specific siRNA. In order to further confirm the specific mechanism of Plin5 in high fat/glucose inducing MCMECs injury, MCMECs in Plin5 siRNA group were divided into vehicle group and N-acetyl cysteine (NA