IMMUNOADSORBENTS FOR THE TREATMENT OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS IN GUINEA PIGS

• Published in: Biotechnologia acta Vol. 5; no. 5; p. 45
• Main Authors: Gurina, N M, Kuchmerovska, T M
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01.09.2012
• Subjects: Acid phosphatase
• ISSN: 1995-5537

The modern presumption of multiple sclerosis as a demyelinating disease of autoimmune nature contributes to the development of biotechnological approaches for removal of autoantibodies, immune complexes and specific clones of CD4^sup +^ T-lymphocytes from the patients systemic circulation for this disease treatment. Preclinical study of new efferent means conducted on laboratory animals with experimental allergic encephalomyelitis. For the treatment of experimental allergic encephalomyelitis in guinea pigs, the authors have used immunosorbents based on oxidized carbon granular matrix KAU and SCN with immobilized myelin basic protein MBP. Experimental allergic encephalomyelitis was induced by single subcutaneous injection of 100 μg of MBP in complete Freund's adjuvantt. It was found that synthesized immunosorbents were in therapeutic efficacy both being used in the latent period of disease and in the presence of its characteristic signs; they enabled to remove up to 37% anti-MBP antibodies from blood serum of guinea pigs with experimental allergic encephalomyelitis and to reduce activity of serum acid phosphatase by 54%.