An Amperometric Immunosensor Based on Graphene Composite Film and Protein a for Chlorpyrifos Detection

In this article, an immunosensor was designed for chlorpyrifos detection, which was based on graphene-multi-walled carbon nanotubes-gold nanoparticle-chitosan nanocomposite film. Protein A can combine with gold nanoparticles, which made anti-chlorpyrifos antibody immobilized orientedly, eventually t...

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Bibliographic Details
Published inSensors & transducers Vol. 178; no. 9; p. 47
Main Authors Wang, Xiangyou, Cao, Yaoyao, Chen, Dongfei, Zhao, Guo, Sun, Xia
Format Journal Article
LanguageEnglish
Published Toronto IFSA Publishing, S.L 01.09.2014
Subjects
Carbon
Chlorpyrifos
Electrodes
Graphene
Immunosensors
Mathematical analysis
Nanocomposites
Nanoparticles
Nanostructure
Protein A
Proteins
Transducers
Voltammetry
China
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Summary:In this article, an immunosensor was designed for chlorpyrifos detection, which was based on graphene-multi-walled carbon nanotubes-gold nanoparticle-chitosan nanocomposite film. Protein A can combine with gold nanoparticles, which made anti-chlorpyrifos antibody immobilized orientedly, eventually the modified immunosensor was developed for the detection of chlorpyrifos residues. Under the optimized conditions, a regression equation: y=9.5676 lgC (ng/mL) +18.164 (R^sup 2^=0.9976) was obtained with a detection limit as low as 0.037 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, it has the potential of real sample detection.
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ISSN:2306-8515
1726-5479