Diversity of bla Ctx-m type genes in Salmonella serovars isolated from raw chicken gizzards in Côte d'Ivoire by sequencing of universal sequence tagged PCR-amplicons

• Published in: Octa journal of biosciences Vol. 4; no. 1
• Main Authors: Bonny Aya Carole, Karou Tago Germain, Ngazoa Kakou Solange, Sébastien, Niamké Lamine
• Format: Journal Article
• Language: English
• Published: Dehradun Scientific Planet Society 01.06.2016
• ISSN: 2321-3663

Multidrug resistance is emerging in many Gram-negative bacteria like Salmonella spp . Plasmid encoding bla -Ctx-m enzymes represent an important sub-group of class A ?-lactamases responsible for ESBL ( Extended-spectrum ?-lactamases) phenotype which is increasingly found in Enterobacteriaceae such as Salmonella spp. Molecular typing of ESBL-isolates has become more important for prevention of ESBL-producers dissemination in environment. bla Ctx-m genes were targeted using degenerated bla Ctx-m consensus primers and PCR amplified from Salmonella strains presenting particular features of multidrug resistance, isolated from chicken gizzards. bla Ctx-m sequences analysis in Salmonella isolates (Kentucky and Muenster), revealed similarities of 96 to 100 % of homology with nucleotides fragments encoding to enzymes type such as bla CTX-M-2,-5,-44,-59,-92,-97 and -131; bla NDM-1 and bla OXY. This identification could only been achieved by sequencing of the PCR-amplicons in other Enterobacteriaceae. PCR-based molecular typing method described here, enables a rapid and reliable molecular identification of bla genes. The principles used in this study could also be applied to any situation in which antimicrobial resistance genes would need to be sequenced.