Ratiometric Activatable Cell-Penetrating Peptides Provide Rapid InVivo Readout of Thrombin Activation

In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localiza...

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Published inAngewandte Chemie International Edition Vol. 52; no. 1; pp. 325 - 330
Main Authors Whitney, Michael, Savariar, Elamprakash N, Friedman, Beth, Levin, Rachel A, Crisp, Jessica L, Glasgow, Heather L, Lefkowitz, Roy, Adams, Stephen R, Steinbach, Paul, Nashi, Nadia, Nguyen, Quyen T, Tsien, Roger Y
Format Journal Article
LanguageEnglish
Published Weinheim Wiley Subscription Services, Inc 02.01.2013
EditionInternational ed. in English
Subjects
Activation
Infrared
Localization
Norleucine
Peptides
Real time
Thrombin
Wavelengths
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Summary:In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201205721