THE CHEMICAL COMPOSITION AND ANTIPROLIFERATIVE ACTIVITY OF FRACTIONS OF THE METHANOL EXTRACT FROM THE BASIDIOMES OF Lactarius pergamenus (Fr.)Fr

• Published in: Biotechnologia acta Vol. 5; no. 1; p. 78
• Main Authors: Panchak, L V, Klyuchivska, O Yu, Tsivinska, M V, Stoika, R S, Lesyk, R B, Antonyuk, V O
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01.01.2012
• Subjects: Inula helenium; Lactarius
• ISSN: 1995-5537

The method of fractionation of the methanol extract from the basidiomes of Lactarius pergamenus mushroom by the organic solvents and chromatography on the silica gel column is described. Identification of substance in the obtained fractions was carried out by mass spectrometry. Antiproliferative activity was defined in the culture of murine leukemia cells of L1210 line. It was found that the fraction containing a substance with yellow-green fluorescence under the UV light possessed the highest antiproliferative activity. Higher fatty acids concomitant and their ethers also play a noticeable role in cytotoxic effect. The toxic effect of trimethylfuranolactarane of L. pergamenus towards the cultured murine leukemia cells of L1210 line was many times weaker as compared to such effect of the alantolactone of the Inula helenium rhizomes. However, trimethylfuranolactarane is more effective cytostatic as compared to alantolactone. Thus, the sesquiterpene substances of the fruit bodies of Lactarius mushrooms are a perspective antiproliferative agents.