BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates

Biomolecular condensates play a key role in organizing RNAs and proteins into membraneless organelles. Bacterial RNP-bodies (BR-bodies) containing the RNA degradosome mRNA decay machinery forms a biomolecular condensate, but the biochemical function of such organization remains poorly defined. Here...

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Published inbioRxiv
Main Authors Al-Husini, Nadra, Tomares, Dylan T, Pfaffenberger, Zechariah, Muthunayake, Nisansala S, Samad, Mohammad A, Zuo, Tiancheng, Bitar, Obaidah, Aretakis, James R, Bharmal, Mohammed Husain M, Gega, Alisa, Biteen, Julie S, Childers, W Seth, Schrader, Jared M
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 05.07.2019
Subjects
Antisense RNA
Condensates
Intermediates
mRNA turnover
Organelles
Permeability
rRNA
tRNA
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Summary:Biomolecular condensates play a key role in organizing RNAs and proteins into membraneless organelles. Bacterial RNP-bodies (BR-bodies) containing the RNA degradosome mRNA decay machinery forms a biomolecular condensate, but the biochemical function of such organization remains poorly defined. Here we define the RNA substrates of BR-bodies through enrichment of the bodies followed by RNA-seq. We find that long, poorly translated mRNAs, small RNAs, and antisense RNAs are the main substrates, while rRNA, tRNA, and other conserved ncRNAs are excluded from these bodies. BR-bodies stimulate the mRNA decay rate of enriched mRNAs, helping to reshape the cellular mRNA pool. We also observe that BR-body formation promotes complete mRNA decay, avoiding the build-up of toxic endo-cleaved mRNA decay intermediates. The combined selective permeability of BR-bodies for both enzymes and substrates together with the stimulation of the sub-steps of mRNA decay provide an effective organization strategy for bacterial mRNA decay.
DOI:10.1101/690628