Optimization of enzymatic hydrolysis of visceral waste proteins of beluga sturgeon Huso huso using Alcalase
Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surface methodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94), the hydrolysis conditions (temperature, time and enzyme activity) wer...
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Published in | International aquatic research Vol. 1; no. 1; p. 31 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Tonekabon
Islamic Azad University, Tonekabon Branch, Vali abad
01.11.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surface methodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94), the hydrolysis conditions (temperature, time and enzyme activity) were optimized. The optimum conditions were: 50°C, 120 min, protease (Alcalase® 2.4 L) activity on 34 AU/kg protein. The hydrolysates of beluga visceral protein have a relatively high protein (66.43%) and low lipid (1.34%) content. The chemical score of the hydrolysate indicated that the amino acid profile of beluga sturgeon hydrolysate fulfills human adult nutritional requirements, with phenylalanine being the first limiting and predominant amino acid in the hydrolysate. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2008-4935 2008-6970 |