Optimization of enzymatic hydrolysis of visceral waste proteins of beluga sturgeon Huso huso using Alcalase

Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surface methodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94), the hydrolysis conditions (temperature, time and enzyme activity) wer...

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Bibliographic Details
Published inInternational aquatic research Vol. 1; no. 1; p. 31
Main Authors Ovissipour, Mahmoudreza, Taghiof, Majid, Motamedzadegan, Ali, Rasco, Barbara, Abbas Esmaeili Molla
Format Journal Article
LanguageEnglish
Published Tonekabon Islamic Azad University, Tonekabon Branch, Vali abad 01.11.2009
Subjects
Acipenser
Amino acids
Enzymatic activity
Enzyme activity
Enzymes
Enzymolysis
Factorial design
Fish
Fish protein hydrolysate
Freshwater fishes
Huso huso
Hydrolysates
Hydrolysis
Lipids
Nutritional requirements
Optimization
Phenylalanine
Proteins
Response surface methodology
Sturgeon
Subtilisin
Viscera
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Summary:Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surface methodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94), the hydrolysis conditions (temperature, time and enzyme activity) were optimized. The optimum conditions were: 50°C, 120 min, protease (Alcalase® 2.4 L) activity on 34 AU/kg protein. The hydrolysates of beluga visceral protein have a relatively high protein (66.43%) and low lipid (1.34%) content. The chemical score of the hydrolysate indicated that the amino acid profile of beluga sturgeon hydrolysate fulfills human adult nutritional requirements, with phenylalanine being the first limiting and predominant amino acid in the hydrolysate.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2008-4935
2008-6970