Changing Times: Fluorescence-lifetime Analysis of Amyloidogenic SF-IAPP Fusion Protein

The fluorescence lifetime of the superfolder green fluorescent protein (SF) and the SF protein fused with islet amyloid polypeptide (SF-IAPP) were studied in polyacrylamide gel. It was shown that the SF average fluorescence lifetime under these conditions slightly differs from that of the SF-IAPP mo...

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Bibliographic Details
Published inbioRxiv
Main Authors Antimonova, Olga I, Lebedev, Dmitry V, Zabrodskaya, Yana A, Grudinina, Natalia A, Shavlovsky, Michael M, Egorov, Vladimir V
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 09.05.2018
Subjects
Amylin
Amyloid
Amyloidogenesis
Fibrils
Fusion protein
Green fluorescent protein
Lifetime
Proteins
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Summary:The fluorescence lifetime of the superfolder green fluorescent protein (SF) and the SF protein fused with islet amyloid polypeptide (SF-IAPP) were studied in polyacrylamide gel. It was shown that the SF average fluorescence lifetime under these conditions slightly differs from that of the SF-IAPP monomer. SF-IAPP does not lose the ability to form amyloid-like fibrils; meanwhile, the average fluorescence lifetime of the fusion protein in fibrils is reduced. We propose the application of Fluorescent-lifetime Imaging Microscopy (FLIM) to the measurement of average fluorescence lifetimes of fusion proteins (amyloidogenic protein-SF) in the context of studies using cellular models of conformational diseases. Footnotes * Misprint in abstract was corrected.
DOI:10.1101/317917