MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magn...

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Bibliographic Details
Published inbioRxiv
Main Author Templier, Thomas
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 26.01.2019
Subjects
Anatomy
Electron microscopy
Embedding
Microscopy
Silicon wafers
Tracers
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Summary:The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.
DOI:10.1101/526137