Single Cell Phenotyping Reveals Heterogeneity among Haematopoietic Stem Cells Following Infection

The haematopoietic stem cell (HSC) niche provides essential micro-environmental cues for the production and maintenance of HSCs within the bone marrow. During inflammation, haematopoietic dynamics are perturbed, but it is not known whether changes to the HSC-niche interaction occur as a result. We v...

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Bibliographic Details
Published inbioRxiv
Main Authors Maclean, Adam L, Smith, Maia A, Liepe, Juliane, Sim, Aaron, Khorshed, Reema, Rashidi, Narges M, Scherf, Nico, Krinner, Axel, Roeder, Ingo, Cristina Lo Celso, Michael Ph Stumpf
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 25.04.2017
Subjects
Bone marrow
Cell adhesion & migration
Cell migration
Computer applications
Hematopoietic stem cells
Infections
Life history
Phenotyping
Statistical analysis
Stem cell transplantation
Stem cells
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Summary:The haematopoietic stem cell (HSC) niche provides essential micro-environmental cues for the production and maintenance of HSCs within the bone marrow. During inflammation, haematopoietic dynamics are perturbed, but it is not known whether changes to the HSC-niche interaction occur as a result. We visualise HSCs directly in vivo, enabling detailed analysis of the 3D niche dynamics and migration patterns in murine bone marrow following Trichinella spiralis infection. Spatial statistical analysis of these HSC trajectories reveals two distinct modes of HSC behaviour: (i) a pattern of revisiting previously explored space, and (ii) a pattern of exploring new space. Whereas HSCs from control donors predominantly follow pattern (i), those from infected mice adopt both strategies. Using detailed computational analyses of cell migration tracks and life-history theory, we show that the increased motility of HSCs following infection can, perhaps counterintuitively, enable mice to cope better in deteriorating HSC-niche micro-environments following infection.
DOI:10.1101/080416