CHCHD10 OR CHCHD2 ARE NOT REQUIRED FOR HUMAN MOTOR NEURON DIFFERENTIATION IN VITRO BUT MODIFY SYNAPTIC TRANSCRIPTOMES

Mitochondrial intermembrane space proteins CHCHD2 and CHCHD10 have roles in diseases affecting motor neurons such as amyotrophic lateral sclerosis, spinal muscular atrophy and axonal neuropathy and in Parkinson's disease, and form a complex of unknown function. Here we address the importance of...

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Published inbioRxiv
Main Authors Harjuhaahto, Sandra, Rasila, Tiina S, Molchanova, Svetlana M, Woldegebriel, Rosa, Kvist, Jouni, Konovalova, Svetlana, Sainio, Markus T, Pennonen, Jana, Ibrahim, Hazem, Otonkoski, Timo, Taira, Tomi, Ylikallio, Emil, Tyynismaa, Henna
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 01.11.2019
Subjects
Amyotrophic lateral sclerosis
Gene expression
Mitochondria
Motor neurons
Movement disorders
Neurodegenerative diseases
Neurons
Neuropathy
Parkinson's disease
Pluripotency
Spinal muscular atrophy
Stem cells
Ultrastructure
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Summary:Mitochondrial intermembrane space proteins CHCHD2 and CHCHD10 have roles in diseases affecting motor neurons such as amyotrophic lateral sclerosis, spinal muscular atrophy and axonal neuropathy and in Parkinson's disease, and form a complex of unknown function. Here we address the importance of these two proteins in human motor neurons. We show that gene edited human induced pluripotent stem cells (iPSC) lacking either CHCHD2 or CHCHD10 are viable and can be differentiated into functional motor neurons that fire spontaneous and evoked action potentials. Knockout iPSC and motor neurons sustain mitochondrial ultrastructure and show reciprocal compensatory increases in CHCHD2 or CHCHD10. Knockout motor neurons have largely overlapping transcriptome profiles compared to isogenic control line, in particular for synaptic gene expression. Our results show that absence of CHCHD2 or CHCHD10 does not disrupt functionality, but induces similar modifications in human motor neurons. Thus pathogenic mechanisms may involve loss of synaptic function.
DOI:10.1101/828376