Determinants of genetic structure of the Sub-Saharan parasitic wasp Cotesia sesamiae

• Published in: bioRxiv
• Main Authors: Branca, Antoine, Le Ru, Bruno P, Paul-Andr Calatayud, Obonyo, Julius O, Musyoka, Boaz, Capdevielle-Dulac, Claire, Kaiser-Arnauld, Laure, Jean-Fran ois Silvain, J r my Gauthier, Paillusson, Corentin, Gayral, Philippe, Herniou, Elisabeth, St phane Dupas
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 26.09.2017
• Subjects: Abiotic factors; Biodiversity; Biogeography; Biological control; Climatic conditions; Gene flow; Genetic markers; Genetic structure; Geography; Host-parasite interactions; Life history; Microsatellites; Specialization; Strains (organisms); Sub-Saharan Africa
• DOI: 10.1101/194084

Parasitoid life style represents one of the most diversified life history strategies on earth. There are however very few studies on the variables associated with intraspecific diversity of parasitoid insects, especially regarding the relationship with spatial, biotic and abiotic ecological factors. Cotesia sesamiae is a Sub-Saharan stenophagous parasitic wasp that parasitizes several African stemborer species with variable developmental success. The different host-specialized populations are infected with different strains of Wolbachia, an endosymbiotic bacterium widespread in arthropods that is known for impacting life history traits notably reproduction, and consequently species distribution. In this study, first we analyzed the genetic structure of C. sesamiae across Sub-Saharan Africa, using 8 microsatellite markers, and 3 clustering software. We identified five major population clusters across Sub-Saharan Africa, which probably originated in East African Rift region and expanded throughout Africa in relation to host genus and abiotic factors such as climatic classifications. Using laboratory lines, we estimated the incompatibility between the different strains of Wolbachia infecting C. sesamiae. We observed an incompatibility between Wolbachia strains was asymmetric; expressed in one direction only. Based on these results, we assessed the relationships between direction of gene flow and Wolbachia infections in the genetic clusters. We found that Wolbachia-induced reproductive incompatibility was less influential than host specialization in the genetic structure. Both Wolbachia and host were more influential than geography and current climatic conditions. These results are discussed in the context of African biogeography, and co-evolution between Wolbachia, virus parasitoid and host, in the perspective of improving biological control efficiency through a better knowledge of the biodiversity of biological control agents.