Focus: The interface between data collection and data processing in cryo-EM

We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image process...

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Bibliographic Details
Published inbioRxiv
Main Authors Biyani, Nikhil, Righetto, Ricardo D, Mcleod, Robert, Caujolle-Bert, Daniel, Castano-Diez, Daniel, Goldie, Kenneth N, Stahlberg, Henning
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.03.2017
Subjects
Automation
Crystals
Data collection
Data processing
Image processing
Interfaces
Transmission electron microscopy
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Summary:We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope.
DOI:10.1101/105452