DNA origami nanostructures as a tool for the targeted destruction of bacteria

Antibiotic resistance is a growing worldwide human health issue that is now rendering us vulnerable once again to infections that have been treatable for decades. Various approaches have been proposed in an effort to overcome this threat and effectively treat bacterial infections. We use a DNA origa...

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Bibliographic Details
Published inbioRxiv
Main Authors Mela, Ioanna, Vallejo-Ramirez, Pedro P, Makarchuk, Stanislaw, Christie, Graham, David Md Bailey, Henderson, Robert M, Sugiyama, Hiroshi, Endo, Masayuki, Kaminski, Clemens F
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 12.12.2019
Subjects
Antibiotic resistance
Antibiotics
Aptamers
Atomic force microscopy
Bacteria
Bacterial infections
Deoxyribonucleic acid
DNA
Drug delivery
Drug resistance
Lysozyme
Microscopy
Stochasticity
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Summary:Antibiotic resistance is a growing worldwide human health issue that is now rendering us vulnerable once again to infections that have been treatable for decades. Various approaches have been proposed in an effort to overcome this threat and effectively treat bacterial infections. We use a DNA origami nanostructure, functionalized with aptamers, as a vehicle for delivering the antibacterial peptide lysozyme in a specific and efficient manner, in order to destroy bacterial targets. We test the system against Gram positive (Bacillus subtilis) and Gram negative (Escherichia coli) targets. We use direct stochastic optical reconstruction microscopy (dSTORM) and atomic force microscopy (AFM) to characterize the DNA origami nanostructures and structured illumination microscopy (SIM) to assess the binding of the origami to the bacteria. We show that treatment with lysozyme-functionalized origami slows bacterial growth more effectively than treatment with free lysozyme. Our study introduces DNA origami as a tool in the fight against antibiotic resistance, and our results demonstrate the specificity and efficiency of the nanostructure as a drug delivery vehicle. Footnotes * Figure 4 - updated
DOI:10.1101/837252