Transient elevation of ribonucleotide reductase activity, M2 mRNA and M2 protein in BALB/c 3T3 fibroblasts in the presence of 12-0-tetradecanoylphorbol-13-acetate

A rapid elevation of ribonucleotide reductase activity was observed with BALB/c 3T3 fibroblasts within 1/2 to 1 hour treatment with 0.1 mu M 12-0-tetradecanoylphorbol-13-acetate (TPA). This increase in activity was transient, and returned to about normal levels within 24 to 48 hours. Northern analys...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 162; no. 3; pp. 1417 - 1424
Main Authors CHOY, B. K, MCCLARTY, G. A, WRIGHT, J. A
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier 1989
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Oxidoreductases
Cell culture
Messenger RNA
Ribonucleotide reductase
Enzymatic activity
Gel electrophoresis
Blotting assay
Tumor promotor
3T3-Cell line
Activation
Fibroblast
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Summary:A rapid elevation of ribonucleotide reductase activity was observed with BALB/c 3T3 fibroblasts within 1/2 to 1 hour treatment with 0.1 mu M 12-0-tetradecanoylphorbol-13-acetate (TPA). This increase in activity was transient, and returned to about normal levels within 24 to 48 hours. Northern analysis of the two components of ribonucleotide reductase showed a slight transient elevation of M1 mRNA and a marked transient elevation of M2 mRNA after 1/2 hour TPA treatment. As a positive control, ornithine decarboxylase message levels were also observed to be transiently elevated following identical treatment with TPA.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0006-291X
1090-2104