Early detection of active Human Cytomegalovirus infection after living-donor liver transplantation

Human cytomegalovirus (HCMV) is a member of the beta herpes virinae. It is one of the most important virus in transplantation. It has direct and indirect impact on liver transplant recipient outcome. We aimed to diagnose early active HCMV infection in living donor liver transplant (LDLT) recipients....

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Bibliographic Details
Published inbioRxiv
Main Authors Mohamed Anies Rizk, Salah Abd Elfatah Agha, Maysaa El Sayed Zaki, Noha Badr El-Deen El-Mashad, Mohamed Mahmoud Fahmy El-Saadany
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 20.04.2018
Subjects
Cytomegalovirus
Enzyme-linked immunosorbent assay
Gastroenterology
Immunoglobulin G
Immunoglobulin M
Infections
Liver
Liver transplantation
Polymerase chain reaction
Transplants & implants
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Summary:Human cytomegalovirus (HCMV) is a member of the beta herpes virinae. It is one of the most important virus in transplantation. It has direct and indirect impact on liver transplant recipient outcome. We aimed to diagnose early active HCMV infection in living donor liver transplant (LDLT) recipients. Also, to correlate the associated clinical and laboratory findings with HCMV infection. Here, we investigate 76 LDLT recipients for early detection of active HCMV infection in a period of 1-6 months after liver transplantation upon their suggested clinical data. These samples were collected in the period from 4/2013 to 12/ 2015 at Gastroenterology center, Mansoura University. They were 68 males and 8 females. HCMV infection diagnosed by ELISA (Ig M, Ig G) and real time PCR. Seventy-four patients were IgG seropositive recipient. Three patients (3.9%) were positive IgM. Ten samples from 76 patients (13.2%) were positive by real-time polymerase chain reaction (PCR). In this study, we concluded that, LDLT recipients are at high risk of HCMV infection (13.2%) and the most suitable method for HCMV detection was PCR.
DOI:10.1101/304360