Inhaled nitric oxide increases surfactant protein gene expression in the intact lamb

Inhaled nitric oxide (iNO) is used to treat a number of disease processes. Although in vitro data suggest that nitric oxide (NO) alters surfactant protein gene expression, the effects in vivo have not been studied. The objective of this study was to evaluate the effects of iNO on surfactant protein...

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Published inAmerican journal of physiology. Lung cellular and molecular physiology Vol. 29; no. 3; pp. L628 - L633
Main Authors STUART, Regan B, OVADIA, Boaz, SUZARA, Vincent V, ROSS, Patrick A, THELITZ, Stephan, FINEMAN, Jeffrey R, GUTIERREZ, Jorge A
Format Journal Article
LanguageEnglish
Published Bethesda, MD American Physiological Society 01.09.2003
Subjects
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Deoxyribonucleic acid
DNA
Emergency and intensive respiratory care
Intensive care medicine
Medical research
Medical sciences
Nitric oxide
Proteins
Respiratory diseases
Respiratory intensive care
Gene expression
Respiratory system
Protein
Vertebrata
Mammalia
Messenger RNA
Pulmonary surfactant
surfactant proteins
Animal
Nitric oxide
Inhaled gas
Sheep
Artiodactyla
Mechanical ventilation
Ungulata
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Summary:Inhaled nitric oxide (iNO) is used to treat a number of disease processes. Although in vitro data suggest that nitric oxide (NO) alters surfactant protein gene expression, the effects in vivo have not been studied. The objective of this study was to evaluate the effects of iNO on surfactant protein (SP)-A, -B, and -C gene expression in the intact lamb. Thirteen 4-wk-old lambs were mechanically ventilated with 21% oxygen and received iNO at 40 ppm (n = 7) or vehicle gas (n = 6) for 24 h. Peripheral lung biopsies were obtained at 0, 12, and 24 h and analyzed for surfactant mRNA, protein, and total DNA content. Inhaled NO increased SP-A and SP-B mRNA content by 80% from 0 to 12 h and by 78 and 71%, respectively, from 0 to 24 h. There was an increase in SP-A and SP-B protein content by 45% from 0 to 12 h, and a decrease by 70 and 65%, respectively, from 0 to 24 h. DNA content was unchanged. The mechanisms and physiological effects of these findings warrant further investigation. [PUBLICATION ABSTRACT]
ISSN:1040-0605
1522-1504