Identification of Q-markers for Schisandrae Sphenantherae Fructus in treating drug-induced liver injury based on network pharmacology, fingerprint and quantitative analysis

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 20; p. 5460
• Main Authors: Lin, Lu-Jie, Zhang, Ming-Xiao, Li, Hua, Lan, Xue-Mei, Wei, Xiao-Lu, Guo, Cong, Yang, Bin
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2023
• Subjects: Chemical and Drug Induced Liver Injury - drug therapy; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Network Pharmacology; Schisandra - chemistry; drug-induced liver injury; fingerprint; Schisandrae Sphenantherae Fructus; network pharmacology; quality markers
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20230627.201

This study aims to establish the ultra-performance liquid chromatography(UPLC) fingerprint and multi-indicator quantitative analysis method for Schisandrae Sphenantherae Fructus(SSF) and to screen out the potential quality markers(Q-markers) of hepatoprotection based on network pharmacology. The similarity analysis was performed using the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System, which showed that the similarity of the fingerprints of 15 samples from different regions ranged from 0.981 to 0.998. Eighteen common components were identified, from which 3 differential components were selected by cluster analysis and principal component analysis. The "component-target-pathway" network was built to predict the core components related to the hepatoprotective effects. Fourteen core components were screened by network pharmacology. They acted on the targets such as AKT1, CCND1, CYP1A1, CYP3A4, MAPK1, MAPK3, NOS2, NQO1, and PTGS2 to regulate the signaling pathways of lipid metabolism an