Effect of Tongdu Tiaoshen acupuncture on PI3K/Akt/mTOR signaling pathway and autophagy-related proteins of hippocampus in rats with post-stroke depression

• Published in: Zhōngguó zhēnjiǔ Vol. 40; no. 11; p. 1205
• Main Authors: Sun, Pei-Yang, Li, Pei-Fang, Wang, Tao, Wu, Jie, Li, Nan, Liu, Hui, Li, A-Hui, Zhang, Jing-Jing, Chu, Hao-Ran
• Format: Journal Article
• Language: Chinese
• Published: China 12.11.2020
• Subjects: Acupuncture Points; Acupuncture Therapy; Animals; Autophagy-Related Proteins; Depression - etiology; Depression - therapy; Hippocampus; Phosphatidylinositol 3-Kinase; Phosphatidylinositol 3-Kinases - genetics; Proto-Oncogene Proteins c-akt; Rats; Rats, Sprague-Dawley; Signal Transduction; Stroke - complications; Stroke - therapy; TOR Serine-Threonine Kinases; PI3K/Akt/mTOR signaling pathway; autophagy; Tongdu Tiaoshen acupuncture; hippocampus; post-stroke depression
• ISSN: 0255-2930
• DOI: 10.13703/j.0255-2930.20200522-k0006

To observe the improvement of acupuncture on depression-like behavior in rats with post-stroke depression (PSD), and to explore its possible mechanism. A total of 48 SD rats were randomized into a sham-operation group, a model group, a Tongdu Tiaoshen group and a non-meridian-non-acupoint group, 12 rats in each group. In the the model group, the Tongdu Tiaoshen group and the non-meridian-non-acupoint group, PSD model was established by the compound method of cerebral medial arterial occlusion, chronic unpredictable mild stress and isolation. Acupuncture was applied at "Dazhui"(GV 14), "Shuigou"(GV 26), "Baihui"(GV 20) and "Shenting" (GV 24) in the Tongdu Tiaoshen group, and at the bilateral dorsum hollows between 3rd and 4th metatarsal bones of the forelegs in the non-meridian-non-acupoint group, needles were retained for 40 min in the both groups, once a day, 6 times a week for 4 weeks. Before model establishment and after intervention, the ethology indice of Zea Longa neurological behavioral score, open-fie