Investigation of membrane permeability of carp spermatozoa for water molecules

The fundamentals of a photometry method for determination of membrane permeability of some fish spermatozoa for water molecules are presented. Osmotic tolerance of carp spermatozoa membranes was studied using EPR-spectroscopy and photometric analysis methods. It was shown that carp spermatozoa look...

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Bibliographic Details
Published inBiofizika Vol. 59; no. 3; p. 481
Main Authors Pugovkin, A Iu, Kopeĭka, E F, Nardid, O A, Cherkashina, Ia O
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2014
Subjects
Animals
Carps - metabolism
Cell Membrane Permeability - physiology
Electron Spin Resonance Spectroscopy
Male
Osmolar Concentration
Osmosis - physiology
Spermatozoa - metabolism
Water - metabolism
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Summary:The fundamentals of a photometry method for determination of membrane permeability of some fish spermatozoa for water molecules are presented. Osmotic tolerance of carp spermatozoa membranes was studied using EPR-spectroscopy and photometric analysis methods. It was shown that carp spermatozoa look like the ideal osmometers in their reaction on media of different osmolarity. The value of membrane permeability of carp spermatozoa for water molecules was determined. Data obtained can be used in cryobiology for creating cryoprotective media and regimes of fish sperm cryopreservation.
ISSN:0006-3029