Effects of Chlorine Dioxide Disinfection on the Profile of the Super Antibiotic Resistance Genes in a Wastewater Treatment Plant

• Published in: Huanjing kexue Vol. 42; no. 4; p. 1939
• Main Authors: Cheng, Chun-Yan, Li, Hai-Bei, Liang, Yong-Bing, Shi, Dan-Yang, Chen, Zheng-Shan, Yang, Dong, Jiao, Qiao-Rui, Shao, Yi-Fan, Li, Jun-Wen, Jin, Min
• Format: Journal Article
• Language: Chinese
• Published: China 08.04.2021
• Subjects: Anti-Bacterial Agents - pharmacology; Chlorine; Chlorine Compounds - pharmacology; Disinfectants - pharmacology; Disinfection; Drug Resistance, Microbial - genetics; Oxides - pharmacology; Water Purification; chlorine dioxide(ClO2); extracellular super antibiotic resistance genes(eSARGs); disinfection; intracellular super antibiotic resistance genes(iSARGs); wastewater treatment plant(WWTP)
• ISSN: 0250-3301
• DOI: 10.13227/j.hjkx.202008182

In order to study the effects of chlorine dioxide (ClO ) disinfection on the super antibiotic resistance genes (SARGs), the final effluents before and after chlorine dioxide were sampled throughout one year in a wastewater treatment plant (WWTP). The bacteria and extracellular nucleic acid were collected using microporous membrane filtration and nucleic acid adsorption particles, respectively. A total of 9 SARGs was detected through a quantitative real-time polymerase chain reaction (qPCR). The results revealed that both intracellular and extracellular , , and could be positively detected in the samples. Overall, ClO disinfection enhanced the relative abundance of the iSARGs ( <0.05), exhibiting a seasonal pattern, and increasing in the spring, summer, and autumn. In spring, it improved the most, up to twice the abundance. No SARGs were detected positive in the winter, either intracellularly or extracellularly. There was no significant variation in the concentrations of eSARGs before and after ClO disinfectio