Mapping and cloning of GAD1-2 for long awn using CSSLs in rice (Oryza sativa L.)

• Published in: Yíchuán Vol. 40; no. 12; p. 1101
• Main Authors: Yang, De Wei, Zheng, Xiang Hua, Cheng, Chao Ping, Ye, Ning, Huang, Feng Huang, Ye, Xin Fu
• Format: Journal Article
• Language: Chinese
• Published: China 20.12.2018
• ISSN: 0253-9772
• DOI: 10.16288/j.yczz.18-044

Rice is an important food crop in the world. The awn may protect rice seeds from being cut by birds, which is important in rice domestication, survival and diffusion. However, the characteristic of awn is gradually washed out during rice domestication and artificial selection. Mapping and cloning of rice awn genes is the basis of studying the genetic mechanism of awn domestication. In this study, 146 chromosome segment substitution lines (CSSLs) derived from DongNanHui 810/ZhangPu wild rice with DongNanHui 810 as the recurrent parent were used to analyze the quantitative trait loci (QTL) controlling the long awn of rice. The results showed that four CSSLs contained one QTL for the long awn. Using substitution mapping, the GAD1-2 gene was mapped between two markers (Ind8-10 and RM4936) on chromosome 8, with a genetic distance of about 4.75 Mb. Using the dominant individuals of segregating populations, the GAD1-2 gene was eventually located between two Indel markers, with a physical distance of about 27 kb, whi