Effects of bisphenol A exposure on proteins tyrosine phosphorylation in mammalian sperm

Bisphenol A (BPA) is a synthetic estrogen compound, which widely exists in the environment, interferes with mammalian endocrine and affects the function of reproductive system of males. Taking fresh sperm of boar, 17 ℃ preservation boar sperm, and mouse sperm as test materials, we examined the effec...

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Published inYing yong sheng tai xue bao Vol. 33; no. 4; p. 1131
Main Authors Hu, Qi-Meng, Li, Shan-Shan, Yang, Yu-Xin, Li, Meng-Yu, He, Jian-Yun, He, Qun
Format Journal Article
LanguageChinese
Published China 01.04.2022
Subjects
BPA
capacitation
sperm
tyrosine phosphorylation
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Summary:Bisphenol A (BPA) is a synthetic estrogen compound, which widely exists in the environment, interferes with mammalian endocrine and affects the function of reproductive system of males. Taking fresh sperm of boar, 17 ℃ preservation boar sperm, and mouse sperm as test materials, we examined the effects of BPA (0, 0.1,1,10,100 μmol∙L ) on proteins tyrosine phosphorylation in sperm and the molecular mechanism by using wes-tern blot (WB) and immunofluorescence techniques coupled to culture method. The results showed that low BPA concentration (0.1, 1 μmol∙L ) markedly accelerated the protein tyrosine phosphorylation of fresh boar capacitated sperm. However, the tyrosine phosphorylation of boar sperm decreased in high BPA concentration (10, 100 μmol∙L ). The tyrosine phosphorylation of the mouse sperm raised with the increases of BPA concentration. Moreover, BPA affected different kinds of proteins related to tyrosine phosphorylation modification of porcine and mouse sperm capacitation, suggesting that the effect
ISSN:1001-9332
DOI:10.13287/j.1001-9332.202206.029