Diffusible signal factor (DSF)-mediated quorum sensing modulates swarming in Xanthomonas albilineans

spp. are plant pathogens known for significantly impacting crop yields. Among them, ( ) is notable for colonizing the xylem and causing sugarcane leaf scald disease. This study employed homologous recombination to mutate quorum sensing (QS) regulatory genes ( ) to investigate their role in pathogeni...

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Published inPhytopathology
Main Authors Li, MeiLin, Bao, YiXue, Chen, WenHan, Li, YiSha, Du, JinXia, Khan, Abdullah, Khan, Muhammad Tahir, Powell, Charles A, Chen, BaoShan, Zhang, MuQing
Format Journal Article
LanguageEnglish
Published United States 27.08.2024
Subjects
Bacterial Pathogens
Molecular
Epidemiology
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Summary:spp. are plant pathogens known for significantly impacting crop yields. Among them, ( ) is notable for colonizing the xylem and causing sugarcane leaf scald disease. This study employed homologous recombination to mutate quorum sensing (QS) regulatory genes ( ) to investigate their role in pathogenicity. Deletions of (Δ ), (Δ ), and (Δ ) led to reduced swarming, growth, and virulence. However, DSF supplementation restored swarming and growth in the Δ mutant. Deleting , , and also reduced twitching motility and affected Type IV Pilus (T4P) expression. Transcriptomic analysis revealed that Δ positively regulates flagellar genes. DSF supplementation in Δ (Δ -DSF) modulated the expression of flagellar, chemotaxis, and T4P genes. These findings elucidate the DSF-mediated swarming pathway in and provide valuable insights into its regulatory mechanisms.
ISSN:0031-949X
DOI:10.1094/PHYTO-03-24-0076-R